Testing the anthelmintic activity of Acacia mangium pods with different levels and incubation times against the nematode worm haemonchus contortus in vitro

Authors

Keywords:

Acacia, Anthelminthic, Haemonchus contortus, In vitro, Tannin

Abstract

This study aimed to evaluate the anthelmintic activity of Acacia mangium pods at varying concentrations and incubation times against the nematode worm Haemonchus contortus in vitro. This study used Acacia mangium pods and applied four treatments: P0 (100% Albendazole, positive control), P2Y (6% Acacia mangium pod infusion), P4Y (12% Acacia mangium pod infusion), and P6 (0.9% NaCl, negative control), with observations made every 60 minutes for 6 hours. The adult female nematode worm Haemonchus contortus test method was used to determine the anthelmintic effect of Acacia mangium pods in vitro. The results from in vitro the screening of adult female Haemonchus contortus indicate that increasing the concentration of Acacia mangium pod infusion significantly increases worm mortality (P < 0.05). Acacia mangium pods contain 18.98% tannin which means the anthelmintic effect can be seen from the mortality of adult worms. Based on the research findings, we conclude that Acacia mangium pods provide an anthelmintic effect against adult female nematode worm Haemonchus contortus, so it can be used in controlling nematode worm infection in the digestive tract of small ruminants.

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